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Reports

2009 Nucleic Acid Amplification Dashboard Series 2 -

09_pic_nucleic_acid_ampCatalog number: 0907NAA
Publication date: July 2009
Company-wide electronic copy: Complimentary

Please inquire about single-user* electronic copy pricing
* single-user pricing is intended for small companies, of 40 or less employees, to access The Life Science Dashboard. Please order these copies directly with Percepta Associates.

Overview

Overview

Nucleic acid amplification is one of the most commonly performed molecular biology techniques and is a necessary precursor to a range of methods from gene cloning and site-directed mutagenesis to the quantitative analysis of gene expression. The Nucleic Acid Amplification Dashboard was developed from responses to a 21-question survey by 485 scientists predominantly located in North America and Europe. This Dashboard reveals key market indicators for the nucleic acid amplification market as a whole as well as for the following sub-segments:

  • Standard PCR with Taq polymerase, hot start Taq polymerase and proofreading polymerases
  • qPCR with Taq polymerase and with hot start Taq polymerase
  • qRT-PCR with Taq polymerase and with hot start Taq polymerase
  • Instruments for Standard and Real Time PCR

While standard protocols may be well-defined, amplification markets are quite fluid, with significant influence from such external sources as:

  • An evolving intellectual property landscape
  • New pricing strategies from established suppliers
  • Application to emerging research areas such as gene silencing, SNP genotyping and rapid, whole genome sequencing

In order to dive more deeply into the characteristics and dynamics of the market for nucleic acid amplification products, Percepta has introduced the Nucleic Acid Amplification Dashboard, designed to take a snapshot of the current market landscape with the future goal of repeating and publishing the study to give Dashboard readers the ongoing story of how the market is adapting to new products, new competitors and new sales and marketing strategies.

Survey Methodology

In June of 2009, Percepta fielded the Nucleic Acid Purification Survey to a subset of the Percepta BioAnalytix™ Panel of life scientists. Individuals were invited by e-mail blast to click-through to a webpage at perceptabioanalytix.com where the survey was hosted. Invitations were delivered beginning on June 1, 2009 and results collected through June 11, 2009. A total of 485 scientists participated in the survey, of which 442 are actively engaged in performing nucleic acid amplification and 12 plan to use nucleic acid amplification methods in the next 12 months. Results based on the aggregate of collected responses are revealed in this Nucleic Acid Amplification Dashboard.

Important Note:

This report only includes analyses related to the research market for nucleic acid amplification products and also includes the market for products used in molecular diagnostic assays.

A NOTE REGARDING ABBREVIATIONS USED IN THIS REPORT

“Standard or endpoint PCR” – may be abbreviated as “PCR” in this report
“Standard or endpoint reverse transcription PCR” – may be abbreviated as RT-PCR.

“Quantitative Real Time PCR” or “Quantitative PCR”– may be abbreviated as “qRT-PCR” and “qPCR” respectively in this report. qRT-PCR refers to amplification of cDNA and/or RNA templates while qPCR refers to amplification of genomic DNA templates. Product accumulation is analyzed during the reaction.

Respondent Demographics

Respondents from the academic, government and commercial market segments are well represented, with 19.0% of respondents employed in an industry setting. 73.9% of respondents are from North America, while 24.9% reside in Europe.
Junior (Lab Technician, Graduate Student), mid level (Post-Doctoral Fellow, Lab Manager) and senior (Professor/PI, Group Leader) scientists are well represented in the data set, with the most cited job titles being Scientist/Senior Scientist (23.7% of respondents), Lab Manager (14.6%), and Professor/PI (14.4%).

A wide variety of scientific areas of specialization is also evident, led by biochemistry (named by 15.1% of respondents as their primary area of expertise), followed by cell biology (14.7%), microbiology/infectious disease/virology (13.6%) and genomics (8.9%).

Small (1-5 scientists), medium (6-20 scientists) and large (>20 scientists) laboratories are well represented: 39.8% of respondents work in labs where 1 to 5 people perform experiments; 45.7% in labs with 6 to 20 experimenters, and the remaining 14.6% in labs with greater than 20 bench scientists.

Table of contents

Table of Contents

  • Figures and Tables
  • Executive Summary
  • Key Findings and Implications
  • Nucleic Acid Amplification Dashboard
  • Nucleic Acid Amplification Market Opportunity Matrix
  • Survey Methodology
  • Survey Invitation Text
  • Respondent Demographics
  • Frequency of Performance of Molecular Biology Techniques
  • Frequency of Performance of Nucleic Acid Amplification
  • Reaction Throughput and Market Growth Rates
  • Respondent’s Stated Price Per Reaction
  • Total Market Size, Market Segment Sizes and Total Market Growth Rate
  • Market Shares by Segment (Share of Mention)
  • Customer Satisfaction and Interest In Switching Suppliers
  • Product Features That Influence Purchasing Decisions
  • Primary and Secondary Downstream Applications
  • Desired Changes to Nucleic Acid Amplification Products
  • Survey Questionnaire

Figures and Tables

  • Figure 1: Respondent’s Place of Employment
  • Figure 2: Respondent’s Country/Region
  • Figure 3: Respondent’s Job Title
  • Figure 4: Respondent’s Areas of Expertise/Specialization
  • Figure 5: Number of Employees in Respondent’s Laboratories
  • Figure 6: Percentage of Respondents Performing Various Techniques at Least a Few Times per Year
  • Figure 7: Percentage of Respondents Performing Nucleic Acid Amplification
  • Figure 8: Percentage of Respondents using Various Nucleic Acid Amplification Techniques in their Laboratory
  • Figure 9: Percentage of Respondents Performing Standard PCR with Taq Polymerase
  • Figure 10: Percentage of Respondents Performing Standard PCR with a Hot Start Taq Polymerase
  • Figure 11: Percentage of Respondents Performing Standard PCR with a Proofreading (High Fidelity) Polymerase
  • Figure 12: Percentage of Respondents Performing qPCR (Genomic DNA Template) Using Taq Polymerase
  • Figure 13: Percentage of Respondents Performing qPCR (Genomic DNA Template) Using Hot Start Taq Polymerase
  • Figure 14: Percentage of Respondents Performing qRT-PCR (cDNA Template) Using Taq Polymerase
  • Figure 15: Percentage of Respondents Performing qRT-PCR (cDNA Template) Using Hot Start Taq Polymerase
  • Figure 16: Respondent’s Primary Supplier for Standard PCR with Taq Polymerase
  • Figure 17: Respondent’s Primary Supplier for Standard PCR with a Hot Start Taq Polymerase
  • Figure 18: Respondent’s Primary Supplier for Standard PCR with a Proofreading (High Fidelity) Polymerase
  • Figure 19: Respondent’s Primary Supplier for qPCR (gDNA Template) Using Taq Polymerase
  • Figure 20: Respondent’s Primary Supplier for qPCR (gDNA Template) Using Hot Start Taq Polymerase
  • Figure 21: Respondent’s Primary Supplier for qRT-PCR (cDNA Template) Using Taq Polymerase
  • Figure 22: Respondent’s Primary Supplier for qRT-PCR (cDNA Template) Using Hot Start Taq Polymerase
  • Figure 23: Respondent’s Primary Supplier of Standard PCR Thermocyclers
  • Figure 24: Respondent’s Primary Supplier of Instruments for Real-Time PCR
  • Figure 25: Percentage of Respondents That Have Switched Suppliers in the Last Six Months
  • Figure 26: Most Important Features of Nucleic Acid Amplification Products
  • Figure 27: Respondent’s Primary Application for End Products from Standard PCR with Taq Polymerase
  • Figure 28: Respondent’s Primary & Secondary Application for End Products from Standard PCR with Taq Polymerase
  • Figure 29: Respondent’s Primary Application for End Products from Standard PCR with a Hot Start Taq Polymerase
  • Figure 30: Respondent’s Primary & Secondary Application for End Products from Standard PCR with a Hot Start Taq Polymerase
  • Figure 31: Respondent’s Primary Application for End Products from Standard PCR with a Proofreading (High Fidelity) Polymerase
  • Figure 32: Respondent’s Primary & Secondary Application for End Products from Standard PCR with a Proofreading (High Fidelity) Polymerase
  • Figure 33: Respondent’s Primary Application for End Products from qPCR (genomic DNA Template) using Taq Polymerase
  • Figure 34: Respondent’s Primary & Secondary Application for End Products from qPCR (genomic DNA Template) using Taq Polymerase
  • Figure 35: Respondent’s Primary Application for End Products from qPCR (genomic DNA Template) using a Hot Start Taq Polymerase
  • Figure 36: Respondent’s Primary & Secondary Application for End Products from qPCR (genomic DNA Template) using a Hot Start Taq Polymerase
  • Figure 37: Respondent’s Primary Application for End Products from qRT-PCR (cDNA Template) using Taq Polymerase
  • Figure 38: Respondent’s Primary & Secondary Application for End Products from qRT-PCR (cDNA Template) using Taq Polymerase
  • Figure 39: Respondent’s Primary Application for End Products from qRT-PCR (cDNA Template) using a Hot Start Taq Polymerase
  • Figure 40: Respondent’s Primary & Secondary Application for End Products from qRT-PCR (cDNA Template) using a Hot Start Taq Polymerase
  • Table 1: Respondent’s Areas of Expertise/Specialization Values for Figure 4
  • Table 2: Frequency of Performance of Various Life Science Techniques
  • Table 3: Frequency of Co-Performance of Various Life Science Techniques
  • Table 4: Frequency of use for Various Nucleic Acid Amplification Techniques
  • Table 5: Frequency of Co-Performance of Life Science Techniques with the Use of Various Nucleic Acid Amplification Products
  • Table 6: Frequency of Use of Nucleic Acid Amplification Products with the Performance of Life Science Techniques
  • Table 7: Median and Average Monthly Throughput for Nucleic Acid Amplification Products
  • Table 8: Percentage of Respondents Performing Various Numbers of Amplification Reactions Per Month by Product Category
  • Table 9: Projected Growth in the Use of Various Nucleic Acid Amplification Product Categories
  • Table 10: Median and Average Price Per Amplification for Nucleic Acid Amplification Products
  • Table 11: Estimated 2009 Global Market Size for Nucleic Acid Amplification Products by Category
  • Table 12: Respondent’s Primary Supplier for Standard PCR with Taq Polymerase by Market Segment
  • Table 13: Respondent’s Primary Supplier for Standard PCR with a Hot Start Taq Polymerase by Market Segment
  • Table 14: Respondent’s Primary Supplier for Standard PCR with a Proofreading (High Fidelity) Polymerase by Market Segment
  • Table 15: Market Share Leaders for Standard PCR Amplification Products
  • Table 16: Respondent’s Primary Supplier for qPCR (Genomic DNA Template) using Taq Polymerase by Market Segment
  • Table 17: Respondent’s Primary Supplier for qPCR (Genomic DNA Template) using Hot Start Taq Polymerase by Market Segment
  • Table 18: Market Share Leaders for qPCR (Genomic DNA) Amplification Products
  • Table 19: Respondent’s Primary Supplier for qRT-PCR (cDNA template) using Taq Polymerase by Market Segment
  • Table 20: Respondent’s Primary Supplier for qRT-PCR (cDNA template) using a Hot Start Taq Polymerase by Market Segment
  • Table 21: Market Share Leaders for RT-PCR (cDNA) Amplification Products
  • Table 22: Respondent’s Primary Supplier for Standard PCR Thermocyclers by Market Segment
  • Table 23: Respondent’s Primary Supplier for Instruments for Standard Real-Time PCR by Market Segment
  • Table 24: Market Share Leaders for Standard & RT-PCR Instruments
  • Table 25: Percentage of Respondents Satisfied with Various Nucleic Acid Amplification Product Categories and Reasons for Interest in Switching
  • Table 26: Percentage of Respondents Satisfied with Nucleic Acid Amplification Product Categories – Comparison to 2007 Nucleic Acid Amplification Dashboard
  • Table 27: Most Important Features of Products for Nucleic Acid Amplification – Comparison to 2007 Nucleic Acid Amplification Dashboard
  • Table 28: Respondent’s Primary & Secondary Applications for End Products from Standard PCR with Taq Polymerase – Comparison to 2007 Nucleic Acid Amplification Dashboard
  • Table 29: Respondent’s Primary & Secondary Applications for End Products from Standard PCR with a Proofreading (High Fidelity) Polymerase – Comparison to 2007 Nucleic Acid Amplification Dashboard
  • Table 30: Respondent’s Primary & Secondary Applications for End Products from qPCR (genomic DNA template) using Taq Polymerase–Comparison to 2007 Nucleic Acid Amplification Dashboard
  • Table 31: Respondent’s Primary & Secondary Applications for End Products from qRT-PCR (cDNA template) using Taq Polymerase– Comparison to 2007 Nucleic Acid Amplification Dashboard

Questionnaire

Available upon request

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