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Reports

2008 Nucleic Acid Separation Dashboard Series 1 -

pic_nucleic_acid_separation_series_1Catalog number: 0811NAS
Publication date: November 2008
Company-wide electronic copy: Complimentary

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Overview

Overview

The separation of nucleic acids is a necessary precursor to a wide range of life science research and diagnostic techniques. Familiar methods such as cloning/subcloning and Southern and Northern blotting frequently rely on the electrophoretic separation of nucleic acid fragments generated by amplification or restriction digestion. Recently emerging methods such as gene silencing and miRNA analyses often hinge on the separation and sizing of short polynucleotides isolated from cell or tissue samples or synthesized in vitro. For some techniques, such as mutation detection or STR analysis, resolution in the range of a few bases may be required. For other methods, like standard cloning or Southern blotting, a resolution in the tens to hundreds of bases may suffice.

Percepta’s 2008 Nucleic Acid Separation Dashboard™ dives deeply into the characteristics of the market for nucleic acid separation products. This Dashboard reveals key market indicators for the nucleic acids separations market as a whole as well as for the following sub-segments:

  • Analysis of amplified DNA (includes AFLP)
  • Analysis of restriction digested DNA (includes RFLP)
  • Analysis of cRNA or total RNA quality
  • Analysis of small RNA molecules
  • Analysis of synthetic DNA or RNA
  • STR analysis
  • HLA typing analysis
  • Pulsed field gel electrophoresis (includes mapping)
  • Mutation detection (includes SSCP, DGGE)

Survey Methodology

In August of 2008, Percepta fielded the 23-question Nucleic Acid Separation Survey to a subset of the company’s panel of life scientists. Individuals were invited by e-mail to click through to a webpage at bioanalytix.com where the survey was hosted. Invitations were delivered on August 19, 2008 and results collected through August 26th. A total of 548 scientists completed the survey, of which 526 are actively engaged in performing nucleic acid separation and 8 plan to perform nucleic acid separation in the next 12 months. Results based on the aggregate of collected responses are revealed in this Nucleic Acid Separation Life Science Dashboard™.

Respondent Demographics

Respondents from the academic, government and commercial market segments are well represented, with 13.6% of respondents employed in an industry setting. 72.5% of respondents are from North America, while 24.8% reside in Europe.

Junior (Lab Tech, Grad Students), mid level (Post-Doc, Lab Manager) and senior (Professor/PI, Group Leader) scientists are well represented in the data set, with the most cited job titles being Professor/Principal Investigator (19.2% of respondents), Scientist/Senior Scientist (17.3% of respondents) and Post-Doctoral Fellow (14.6%).

A wide variety of scientific areas of specialization is also evident, led by Molecular Biology (named by 34.9% of respondents as their primary area of expertise) and Microbiology/Infectious Disease/Virology (named by 9.9% of respondents). Biochemistry (9.7%), Genetics (8.6%), and Cell Biology (7.3%) are the only other primary areas of specialization named by more than 6% of respondents.

Small (1-5 scientists), medium (6-20 scientists) and large (>20 scientists) laboratories are well represented: 39.6% of respondents work in labs where 1 to 5 people perform experiments; 47.1% in labs with 6 to 20, and the remaining 13.3% in labs with greater than 20 bench scientists.

Table of contents

Table of Contents

  • 6 Figures and Tables
  • 11 Executive Summary
  • 13 Key Findings and Implications
  • 17 Nucleic Acid Separation Dashboard
  • 20 Survey Methodology
  • 22 Survey Invitation Text
  • 23 Respondent Demographics
  • 36 Frequency of Performance of Life Science Techniques
  • 41 Frequency of Performance of Various Nucleic Acid Separation Techniques
  • 58 Reaction Throughput and Market Segment Growth Rates
  • 64 Respondent’s Stated Price Per Reaction
  • 67 Total Market Size, Market Segment Sizes and Total Market Growth Rate
  • 71 Matrix/Method Used to Separate Various Types of Nucleic Acids
  • 84 Market Shares by Segment (Share of Mention)
  • 115 Required Resolution for Nucleic Acid Separation Experiments
  • 128 Required Throughput for Nucleic Acid Separation Experiments
  • 141 Time Required for Nucleic Acid Separation Experiments
  • 154 Customer Satisfaction and Interest in Switching Suppliers
  • 158 Product Features that Influence Purchasing Decisions
  • 162 Desired Changes to Nucleic Acid Separation Products
  • 174 Survey Questionnaire

Figures and Tables

  • 25 Figure 1: Respondent’s Place of Employment
  • 27 Figure 2: Respondent’s Country/Region
  • 29 Figure 3: Respondent’s Position/Job Title
  • 31 Figure 4A: Respondent’s Areas of Expertise/Specialization
  • 32 Figure 4B: Respondent’s Areas of Expertise/Specialization (Molecular Biology Excluded)
  • 35 Figure 5: Number of Employees in Respondent’s Laboratories
  • 38 Figure 6: Percentage of Respondents Performing Various Life Science Techniques at Least a Few Times per Year
  • 43 Figure 7: Percentage of Respondents Performing Nucleic Acid Separation
  • 44 Figure 8: Percentage of Respondents Performing Various Nucleic Acid Separation Methods at Least a Few Times per Year
  • 46 Figure 9: Percentage of Respondents that Analyze Amplified DNA (Includes AFLP)
  • 47 Figure 10: Percentage of Respondents that Analyze Restriction Digested DNA (Includes RFLP)
  • 48 Figure 11: Percentage of Respondents that Analyze cRNA or Total RNA Quality
  • 49 Figure 12: Percentage of Respondents that Analyze Small RNA Molecules
  • 50 Figure 13: Percentage of Respondents that Analyze Synthetic DNA or RNA
  • 51 Figure 14: Percentage of Respondents that Perform STR Analysis
  • 52 Figure 15: Percentage of Respondents that Perform HLA Typing Analysis
  • 53 Figure 16: Percentage of Respondents that Perform Pulsed Field Gel Electrophoresis (Includes Mapping)
  • 54 Figure 17: Percentage of Respondents that Perform Mutation Detection (Includes SSCP, DGGE)
  • 74 Figure 18: Method/Matrix Used by Respondents that Analyze Amplified DNA (Includes AFLP)
  • 75 Figure 19: Method/Matrix Used by Respondents that Analyze Restriction Digested DNA (Includes RFLP)
  • 76 Figure 20: Method/Matrix Used by Respondents that Analyze cRNA or Total RNA Quality
  • 77 Figure 21: Method/Matrix Used by Respondents that Analyze Small RNA Molecules
  • 78 Figure 22: Method/Matrix Used by Respondents that Analyze Synthetic DNA or RNA
  • 79 Figure 23: Method/Matrix Used by Respondents that Perform STR Analysis
  • 80 Figure 24: Method/Matrix Used by Respondents that Perform HLA Typing Analysis
  • 81 Figure 25: Method/Matrix Used by Respondents that Perform Pulsed Field Gel Electrophoresis (Includes Mapping)
  • 82 Figure 26: Method/Matrix Used by Respondents that Perform Mutation Detection (Includes SSCP, DGGE)
  • 88 Figure 27: Respondent’s Primary Supplier of Consumables for Pour-Your-Own Agarose Gels
  • 90 Figure 28: Respondent’s Primary Supplier of Instruments Used to Run Pour-Your-Own Agarose Gels
  • 94 Figure 29: Respondent’s Primary Supplier of Precast Agarose Gels
  • 96 Figure 30: Respondent’s Primary Supplier of Instruments Used to Run Precast Agarose Gels
  • 99 Figure 31: Respondent’s Primary Supplier of Consumables for Separation of Nucleic Acids by Capillary Electrophoresis
  • 101 Figure 32: Respondent’s Primary Supplier of Capillary Electrophoresis Instrumentation for Separation of Nucleic Acids
  • 104 Figure 33: Respondent’s Primary Supplier of Consumables for Microfluidics-Based Separation of Nucleic Acids
  • 106 Figure 34: Respondent’s Primary Supplier of Instrumentation for Microfluidics-Based Separation of Nucleic Acids
  • 109 Figure 35: Respondent’s Primary Supplier of Consumables Used to Run Polyacrylamide Gels
  • 111 Figure 36: Respondent’s Primary Supplier of Instruments Used to Run Polyacrylamide Gels
  • 118 Figure 37: Resolution Required by Respondents that Analyze Amplified DNA (Includes AFLP)
  • 119 Figure 38: Resolution Required by Respondents that Analyze Restriction Digested DNA (Includes RFLP)
  • 120 Figure 39: Resolution Required by Respondents that Analyze cRNA or Total RNA Quality
  • 121 Figure 40: Resolution Required by Respondents that Analyze Small RNA Molecules
  • 122 Figure 41: Resolution Required by Respondents that Analyze Synthetic DNA or RNA
  • 123 Figure 42: Resolution Required by Respondents that Perform STR Analysis
  • 124 Figure 43: Resolution Required by Respondents that Perform HLA Typing Analysis
  • 125 Figure 44: Resolution Required by Respondents that Perform Pulsed Field Gel Electrophoresis (Includes Mapping)
  • 126 Figure 45: Resolution Required by Respondents that Perform Mutation Detection (Includes SSCP, DGGE)
  • 131 Figure 46: Separation Throughput Required by Respondents that Analyze Amplified DNA (Includes AFLP)
  • 132 Figure 47: Separation Throughput Required by Respondents that Analyze Restriction Digested DNA (Includes RFLP)
  • 133 Figure 48: Separation Throughput Required by Respondents that Analyze cRNA or Total RNA Quality
  • 134 Figure 49: Separation Throughput Required by Respondents that Analyze Small RNA Molecules
  • 135 Figure 50: Separation Throughput Required by Respondents that Analyze Synthetic DNA or RNA
  • 136 Figure 51: Separation Throughput Required by Respondents that Perform STR Analysis
  • 137 Figure 52: Separation Throughput Required by Respondents that Perform HLA Typing Analysis
  • 138 Figure 53: Separation Throughput Required by Respondents that Perform Pulsed Field Gel Electrophoresis (Includes Mapping)
  • 139 Figure 54: Separation Throughput Required by Respondents that Perform Mutation Detection (Includes SSCP, DGGE)
  • 144 Figure 55: Completion Time Required by Respondents that Analyze Amplified DNA (Includes AFLP)
  • 145 Figure 56: Completion Time Required by Respondents that Analyze Restriction Digested DNA (Includes RFLP)
  • 146 Figure 57: Completion Time Required by Respondents that Analyze cRNA or Total RNA Quality
  • 147 Figure 58: Completion Time Required by Respondents that Analyze Small RNA Molecules
  • 148 Figure 59: Completion Time Required by Respondents that Analyze Synthetic DNA or RNA
  • 149 Figure 60: Completion Time Required by Respondents that Perform STR Analysis
  • 150 Figure 61: Completion Time Required by Respondents that Perform HLA Typing Analysis
  • 151 Figure 62: Completion Time Required by Respondents that Perform Pulsed Field Gel Electrophoresis (Includes Mapping)
  • 152 Figure 63: Completion Time Required by Respondents that Perform Mutation Detection (Includes SSCP, DGGE)
  • 157 Figure 64: Percentage of Respondents That Have Switched Suppliers in the Last Six Months
  • 160 Figure 65: Most Important Features of Products for Nucleic Acid Separation Experiments
  • 33 Table 1: Respondent’s Areas of Expertise/Specialization – Values for Figures 4A and 4B
  • 39 Table 2: Frequency of Performance of Various Life Science Techniques
  • 40 Table 3: Frequency of Co-Performance of Various Life Science Techniques
  • 45 Table 4: Frequency of Performance of Nucleic Acid Separation Methods
  • 56 Table 5: Frequency of Co-Performance of Life Science Techniques with Nucleic Acid Separation Methods
  • 57 Table 6: Frequency of Co-Performance of Nucleic Acid Separation Methods with Life Science Techniques
  • 60 Table 7: Median and Average Monthly Throughput for Nucleic Acid Separation Techniques
  • 61 Table 8: Percentage of Respondents Separating Various Numbers of Nucleic Acid Samples (Lanes) Per Month
  • 63 Table 9: Projected Growth in the Performance of Various Nucleic Acid Separation Techniques
  • 66 Table 10: Median and Average Price Per Sample for Nucleic Acid Separation Products
  • 69 Table 11: Estimated 2008 Global Market Size for Nucleic Acid Separation Product Categories
  • 70 Table 12: Estimated 2009 Global Market Size for Nucleic Acid Separation Product Categories
  • 83 Table 13: Matrix/Method Used to Separate Various Types of Nucleic Acids
  • 89 Table 14: Market Share Leaders for Consumables for Pour-Your-Own Agarose Gels by Market Segment
  • 91 Table 15: Market Share Leaders for Instruments for Pour-Your-Own Agarose Gels by Market Segment
  • 95 Table 16: Market Share Leaders for Consumables for Precast Agarose Gels by Market Segment
  • 97 Table 17: Market Share Leaders for Instruments for Precast Agarose Gels by Market Segment
  • 100 Table 18: Market Share Leaders for Consumables for Capillary Electrophoresis by Market Segment
  • 102 Table 19: Market Share Leaders for Instruments for Capillary Electrophoresis by Market Segment
  • 105 Table 20: Market Share Leaders for Consumables for Microfluidics-Based Separation of Nucleic Acids by Market Segment
  • 107 Table 21: Market Share Leaders for Instruments for Microfluidics-Based Separation of Nucleic Acids by Market Segment
  • 110 Table 22: Market Share Leaders for Consumables for Polyacrylamide Gels by Market Segment
  • 112 Table 23: Market Share Leaders for Instruments for Polyacrylamide Gels by Market Segment
  • 114 Table 24: Market Share Leaders for Nucleic Acid Separation Products
  • 127 Table 25: Resolution Required for Separation of Various Nucleic Acid Separation Methods
  • 140 Table 26: Throughput Required for Various Nucleic Acid Separation Methods
  • 153 Table 27: Completion Time Required for Various Nucleic Acid Separation Methods
  • 156 Table 28: Percentage of Respondents Satisfied with Various Nucleic Acid Separation Products and Reasons for Dissatisfaction
  • 161 Table 29: Most Important Features of Products for Nucleic Acid Separation Experiments

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