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Reports

2007 Nucleic Acid Amplification Dashboard Series 1 -

pic_nucleic_acid_ampCatalog number: 0701NAA
Publication date: January 2007
Company-wide electronic copy: Complimentary

Please enquire about single-user* electronic copy pricing
* single-user pricing is intended for small companies, of 40 or less employees, to access The Life Science Dashboard. Please order these copies directly with Percepta Associates.

Overview

Overview

Nucleic acid amplification is one of the most commonly performed molecular biology techniques and is a necessary precursor to a range of methods from gene cloning and site-directed mutagenesis to the quantitative analysis of gene expression. The Nucleic Acid Amplification Dashboard was developed from responses to a 37-question survey by 437 scientists predominantly located in North America and Europe. This Dashboard reveals key market indicators for the nucleic acid amplification market as a whole as well as for the following sub-segments:

  • Standard PCR with Taq polymerase, hot start Taq polymerase and proofreading polymerases
  • PCR with Taq polymerase and with hot start Taq polymerase
  • RT-PCR with Taq polymerase and with hot start Taq polymerase.

While standard protocols may be well-defined, amplification markets are quite fluid, with significant influence from such external sources as:

  • an evolving intellectual property landscape
  • new pricing strategies from established suppliers
  • application to emerging research areas such as gene silencing, SNP genotyping and rapid, whole genome sequencing

In order to dive more deeply into the characteristics and dynamics of the market for nucleic acid amplification products, Percepta has introduced the Nucleic Acid Amplification Dashboard, designed to take a snapshot of the current market landscape.

Survey Methodology

In November/December of 2006, Percepta fielded three surveys to the company’s panel of research scientists:

  • Nucleic Acid Purification and Amplification Survey
  • Gene Silencing and Gene Expression Profiling Survey
  • Mammalian Transfection and Cell Culture Survey

This report reveals the results of the Amplification section of the Nucleic Acid Purification and Amplification Survey. The results of the Nucleic Acid Purification section as well as the other two survey projects are available as separate Life Science Dashboard™ publications. Contact Percepta for more information on these additional reports.

Individuals were invited by e-mail blast to click through to a webpage at bioanalytix.com where the survey was hosted. Invitations were delivered on November 13, 2006 and results collected through December 8. A total of 437 scientists completed the survey, of which 418 are actively engaged in the amplification of nucleic acids.

Respondent Demographics

Respondents from the academic, government and commercial market segments are well represented, with nearly 18% of respondents employed in an industry setting. About 75% of respondents are from North America, while 20% reside in Europe.

Junior (Lab Tech, Grad Students), mid level (Post-Doc, Lab Manager) and senior (Professor/PI, Group Leader) scientists are well represented in the data set, with the most cited job titles being Scientist/Senior Scientist (24.0% of respondents) and Lab Manager (13.6%).

A wide variety of scientific areas of specialization is also evident, led by cell biology and biochemistry (each named by 14.6% of respondents as their primary area of expertise), microbiology/ infectious disease/ virology (13.2%) and genomics (9.6%).

Small (1-5 scientists), medium (6-20 scientists) and large (>20 scientists) laboratories are well represented: 34.8% of respondents work in labs where 1 to 5 people perform experiments; 49.7% in labs with 6 to 20 experimenters, and the remaining 16.0% in labs with greater than 20 bench scientists.

Table of contents

Table of Contents

  • Figures and Tables
  • Executive Summary
  • Key Findings and Implications
  • Nucleic Acid Amplification Dashboard
  • Nucleic Acid Amplification Market Opportunity Matrix
  • Survey Methodology
  • Survey Invitation Text
  • Respondent Demographics
  • Frequency of Performance of Molecular Biology Techniques
  • Frequency of Performance of Nucleic Acid Amplification
  • Reaction Throughput and Market Growth Rates
  • Respondent’s Stated Price Per Reaction
  • Total Market Size, Market Segment Sizes and Total Market Growth Rate
  • Market Shares for Products for Standard PCR
  • Market Shares for Products for qPCR with Genomic DNA Templates
  • Market Shares for Products for qRT-PCR with cDNA Templates
  • Market Shares for Instruments for Standard PCR and Real-Time PCR
  • Customer Satisfaction And Interest In Switching Suppliers
  • Product Features That Influence Purchasing Decisions
  • Primary and Secondary Downstream Applications
  • Desired Changes to Nucleic Acid Amplification Products
  • Survey Questionnaire

Figures and Tables

  • Figure 1: Respondent’s Place of Employment
  • Figure 2: Respondent’s Country/Region
  • Figure 3: Respondent’s Job Title
  • Figure 4: Respondent’s Areas of Expertise/Specialization
  • Figure 5: Number of Employees in Respondent’s Laboratories
  • Figure 6: Percentage of Respondents Performing Various Techniques at Least a Few Times per Year
  • Figure 7: Percentage of Respondents Performing Nucleic Acid Amplification
  • Figure 8: Percentage of Respondents Performing Various Nucleic Acid Amplification Techniques at Least a Few Times per Year
  • Figure 9: Percentage of Respondents Performing Standard PCR with Taq Polymerase
  • Figure 10: Percentage of Respondents Performing Standard PCR with a Hot Start Taq Polymerase
  • Figure 11: Percentage of Respondents Performing Standard PCR with a Proofreading (High Fidelity) Polymerase
  • Figure 12: Percentage of Respondents Performing qPCR (Genomic DNA Template) Using Taq Polymerase
  • Figure 13: Percentage of Respondents Performing qPCR (Genomic DNA Template) Using Hot Start Taq Polymerase
  • Figure 14: Percentage of Respondents Performing qRT-PCR (cDNA Template) Using Taq Polymerase
  • Figure 15: Percentage of Respondents Performing qRT-PCR (cDNA Template) Using Hot Start Taq Polymerase
  • Figure 16: Respondent’s Primary Supplier for Standard PCR with Taq Polymerase
  • Figure 17: Respondent’s Primary Supplier for Standard PCR with Taq Polymerase by Region
  • Figure 18: Respondent’s Primary Supplier for Standard PCR with Taq Polymerase by Organization Type
  • Figure 19: Respondent’s Primary Supplier for Standard PCR with a Hot Start Taq Polymerase
  • Figure 20: Respondent’s Primary Supplier for Standard PCR with a Hot Start Taq Polymerase by
  • Figure 21: Respondent’s Primary Supplier for Standard PCR with a Hot Start Taq Polymerase by Organization Type
  • Figure 22: Respondent’s Primary Supplier for Standard PCR with a Proofreading (High Fidelity) Polymerase
  • Figure 23: Respondent’s Primary Supplier for Standard PCR with a Proofreading (High Fidelity) Polymerase by Region
  • Figure 24: Respondent’s Primary Supplier for Standard PCR with a Proofreading (High Fidelity) Polymerase by Organization Type
  • Figure 25: Respondent’s Primary Supplier for qPCR (gDNA Template) Using Taq Polymerase
  • Figure 26: Respondent’s Primary Supplier for qPCR (gDNA Template) Using Taq Polymerase by Region
  • Figure 27: Respondent’s Primary Supplier for qPCR (gDNA Template) Using Taq Polymerase by Organization Type
  • Figure 28: Respondent’s Primary Supplier for qPCR (gDNA Template) Using Hot Start Taq Polymerase
  • Figure 29: Respondent’s Primary Supplier for qPCR (gDNA Template) Using Hot Start Taq Polymerase by Region
  • Figure 30: Respondent’s Primary Supplier for qPCR (gDNA Template) Using Hot Start Taq Polymerase by Organization Type
  • Figure 31: Respondent’s Primary Supplier for qRT-PCR (cDNA Template) Using Taq Polymerase
  • Figure 32: Respondent’s Primary Supplier for qRT-PCR (cDNA Template) Using Taq Polymerase by Region
  • Figure 33: Respondent’s Primary Supplier for qRT-PCR (cDNA Template) Using Taq Polymerase by Organization Type
  • Figure 34: Respondent’s Primary Supplier for qRT-PCR (cDNA Template) Using Hot Start Taq Polymerase
  • Figure 35: Respondent’s Primary Supplier for qRT-PCR (cDNA Template) Using Hot Start Taq Polymerase by Region
  • Figure 36: Respondent’s Primary Supplier for qRT-PCR (cDNA Template) Using Hot Start Taq Polymerase by Organization Type
  • Figure 37: Respondent’s Primary Supplier of Instruments for Standard PCR
  • Figure 38: Respondent’s Primary Supplier of Instruments for Standard PCR by Region
  • Figure 39: Respondent’s Primary Supplier of Instruments for Standard PCR by Organization Type
  • Figure 40: Respondent’s Primary Supplier of Instruments for Real-Time PCR Respondent’s
  • Figure 41: Respondent’s Primary Supplier of Instruments for Real-Time PCR by Region
  • Figure 42: Respondent’s Primary Supplier of Instruments for Real-Time PCR by Organization Type
  • Figure 43: Percentage of Respondents That Have Switched Suppliers in the Last Six Months
  • Figure 44: Most Important Features of Nucleic Acid Amplification Products
  • Figure 45: Respondent’s Primary Application for End Products from Standard PCR
  • Figure 46: Respondent’s Primary and Secondary Applications for End Products from Standard PCR
  • Figure 47: Respondent’s Primary Application for End Products from Standard PCR with a Proofreading Polymerase
  • Figure 48: Respondent’s Primary and Secondary Applications for End Products from Standard PCR with a Proofreading Polymerase
  • Figure 49: Respondent’s Primary Application for End Products from qPCR with a Genomic DNA Template
  • Figure 50: Respondent’s Primary and Secondary Applications for End Products from qPCR with a Genomic DNA Template
  • Figure 51: Respondent’s Primary Application for End Products from qRT-PCR with a cDNA Template
  • Figure 52: Respondent’s Primary and Secondary Applications for End Products from qRT-PCR with a cDNA Template
  • Table 1: Frequency of Performance of Various Molecular Biology Techniques
  • Table 2: Frequency of Co-Performance of Various Molecular Biology Techniques
  • Table 3: Frequency of Performance of Various Nucleic Acid Amplification Methods
  • Table 4: Frequency of Co-Performance of Molecular Biology Techniques with Nucleic Acid Amplification Methods
  • Table 5: Frequency of Co-Performance of Nucleic Acid Amplification Methods with Molecular Biology Techniques
  • Table 6: Median and Average Monthly Throughput for Nucleic Acid Amplification Methods
  • Table 7: Percentage of Respondents Performing Various Numbers of Amplification Reactions Per Month for Nucleic Acid Amplification Methods
  • Table 8: Projected Growth in the Performance of Various Nucleic Acid Amplification Methods
  • Table 9: Median and Average Price Per Prep for Nucleic Acid Amplification Products
  • Table 10: Market Share Leaders for Standard PCR Amplification Products
  • Table 11: Market Share Leaders for qPCR (Genomic DNA) Amplification Products
  • Table 12: Market Share Leaders for qRT-PCR (cDNA Template) Amplification Products
  • Table 13: Market Share Leaders for Instruments for Standard PCR and for Real-Time PCR
  • Table 14: Percentage of Respondents Satisfied with Various Nucleic Acid Amplification Techniques and Reasons for Dissatisfaction

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