Table of Contents

6 Figures and Tables
9 Executive Summary
12 Key Findings and Implications
17 Gene Silencing Dashboard
21 Gene Silencing Market Opportunity Matrix
23 Survey Methodology
25 Survey Invitation Text
26 Respondent Demographics
39 Frequency of Performance of Life Science Techniques
44 Frequency of Performance of Gene Silencing Techniques
63 Reaction Throughput and Market Segment Growth Rates
70 Respondent’s Stated Price per Reaction
73 Total Market Size, Market Segment Sizes and Total Market Growth Rate
75 Market Shares by Segment (Share of Mention)
102 Customer Satisfaction and Interest In Switching Suppliers
108 Product Features That Influence Purchasing Decisions
112 Primary Downstream Applications
129 Desired Changes to Gene Silencing Products
136 Survey Questionnaire

Figures and Tables
28 Figure 1: Respondent’s Place of Employment
30 Figure 2: Respondent’s Country/Region
32 Figure 3: Respondent’s Job Title
34 Figure 4A: Respondent’s Areas of Expertise/Specialization
35 Figure 4B: Respondent’s Areas of Expertise/Specialization (Molecular Biology
      Excluded)
38 Figure 5: Number of Employees in Respondent’s Laboratories
41 Figure 6: Percentage of Respondents Performing Various Life Science
      Techniques at Least a Few Times per Year
46 Figure 7: Percentage of Respondents Performing RNAi/siRNA Experiments
47 Figure 8: Percentage of Respondents Performing Various Gene
      Silencing Techniques at Least a Few Times per Year
49 Figure 9: Percentage of Respondents That Perform RNAi Screens
      Using siRNA Libraries
50 Figure 10: Percentage of Respondents That Induce siRNAs in vivo from
      a Plasmid or Other Vector in Cells
51 Figure 11: Percentage of Respondents That Digest Long dsRNA in vivo
      using Dicer or other Enzymes
52 Figure 12: Percentage of Respondents that Express siRNA in vitro for
      Delivery into Cells
53 Figure 13: Percentage of Respondents that Use PCR Mediated
      siRNA Expression Cassettes to Generate siRNAs
54 Figure 14: Percentage of Respondents that Deliver Synthetic siRNAs
      into Cells (Instrument Mediated)
55 Figure 15: Percentage of Respondents that Deliver Synthetic siRNAs into
      Cells (Lipid Mediated)
56 Figure 16: Percentage of Respondents that Deliver Plasmids into Cells
      for Plasmid Based Gene Knockdown (Instrument Mediated)
57 Figure 17: Percentage of Respondents that Deliver Plasmids into Cells
      for Plasmid Based Gene Knockdown (Lipid Mediated)
58 Figure 18: Percentage of Respondents that Deliver Viral Vectors into Cells
      for Vector Based Gene Knockdown
59 Figure 19: Percentage of Respondents that Use a Reporter Systems to
      Track Gene Knockdown
79 Figure 20: Respondent’s Primary Supplier of Products for Performing
      RNAi Screens Using siRNA Libraries
81 Figure 21: Respondent’s Primary Supplier of Products to Induce siRNAs
      in vivo from a Plasmid or Other Vector in Cells
83 Figure 22: Respondent’s Primary Supplier of Products to Digest Long
      dsRNA in vivo using Dicer or Other Enzymes
85 Figure 23: Respondent’s Primary Supplier of Products to Express siRNA
      in vitro for Delivery into Cells
87 Figure 24: Respondent’s Primary Supplier of PCR Mediated siRNA
      Expression Cassettes to Generate siRNAs
89 Figure 25: Respondent’s Primary Supplier of Products to Deliver Synthetic
      siRNAs into Cells (Instrument Mediated)
91 Figure 26: Respondent’s Primary Supplier of Products to Deliver
      Synthetic siRNAs into Cells (Lipid Mediated)
93 Figure 27: Respondent’s Primary Supplier of Products to Deliver Plasmids into
      Cells for Plasmid Based Gene Knockdown (Instrument Mediated)
95 Figure 28: Respondent’s Primary Supplier of Products to Deliver Plasmids
      into Cells for Plasmid Based Gene Knockdown (Lipid Mediated)
97 Figure 29: Respondent’s Primary Supplier of Products for Viral Delivery of
      Vectors into Cells for Vector Based Gene Knockdown
99 Figure 30: Respondent’s Primary Supplier of Reporter Systems to Track
      Gene Knockdown
107 Figure 31: Percentage of Respondents That Have Switched Suppliers
        in the Last Six Months
110 Figure 32: Most Important Features of Products for Gene
        Silencing Experiments
115 Figure 33: Respondent’s Primary Downstream Application for RNAi
        Screens Using siRNA Libraries
116 Figure 34: Respondent’s Primary Downstream Application for Products
        to Induce siRNAs in vivo from a Plasmid or Other Vector in Cells
117 Figure 35: Respondent’s Primary Downstream Application for Products
        to Digest Long dsRNA in vivo using Dicer or Other Enzymes
118 Figure 36: Respondent’s Primary Downstream Application for Products
        to Express siRNA in vitro for Delivery into Cells
119 Figure 37: Respondent’s Primary Downstream Application for PCR
        Mediated siRNA Expression Cassettes to Generate siRNAs
120 Figure 38: Respondent’s Primary Downstream Application for Products to
        Deliver Synthetic siRNAs into Cells (Instrument Mediated)
121 Figure 39: Respondent’s Primary Downstream Application for Products to
        Deliver Synthetic siRNAs into Cells (Lipid Mediated)
122 Figure 40: Respondent’s Primary Downstream Application for Products to
        Deliver Plasmids into Cells for Gene Knockdown (Instrument Mediated)
123 Figure 41: Respondent’s Primary Downstream Application for Products to
        Deliver Plasmids into Cells for Gene Knockdown (Lipid Mediated)
124 Figure 42: Respondent’s Primary Downstream Application for Products to
        Deliver Vectors into Cells for Vector Based Gene Knockdown
125 Figure 43: Respondent’s Primary Downstream Application for Reporter
        Systems to Track Gene Knockdown

36 Table 1: Respondent’s Areas of Expertise/Specialization Values for
      Figures 4A and 4B
42 Table 2: Frequency of Performance of Various Techniques
43 Table 3: Frequency of Co-Performance of Various Molecular
      Biology Techniques
48 Table 4: Frequency of Performance of Gene Silencing Methods
61 Table 5: Frequency of Co-Performance of Life Science Techniques with
      Gene Silencing Methods
62 Table 6: Frequency of Co-Performance of Gene Silencing Methods with
      Life Science Techniques
65 Table 7: Median and Average Monthly Throughput for Gene Silencing Products
66 Table 8: Percentage of Respondents Performing Various Numbers of Gene
      Silencing Reactions Per Month
67 Table 9: Comparison of to 2007 Percentage of Respondents Performing
      Various Numbers of Gene Silencing Reactions Per Month
69 Table 10: Projected Growth in the Performance of Various Gene
      Silencing Techniques
72 Table 11: Median and Average Price Per Prep for Gene Silencing Products
101 Table 12: Market Share Leaders for Gene Silencing Products
104 Table 13: Percentage of Respondents Satisfied with Various Gene
        Silencing Products and Reasons for Dissatisfaction
105 Table 14: Percentage of Respondents Satisfied with Various Gene
        Silencing Products: Comparison to 2007 Dashboard
111 Table 15: Most Important Features of Products for Gene Silencing
        Experiments – Comparison to 2007 Gene Silencing Dashboard
126 Table 16: Respondents Primary Application After Various Gene
        Silencing Methods
128 Table 17: Respondents Primary Application After Various Gene
        Silencing Methods – Comparison to 2007 Gene Silencing Dashboard